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DNA extraction is a pH-sensitive process, and using a tris buffer helps keep the pH stable over cell lysis and extraction. Tris as a Buffer As pH can influence and be influenced by a number of cellular factors, maintaining a stable pH is essential to experimental science.

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molecules. Ethanol, added to an aqueous DNA solution, disrupts the electrostatic interactions between the water and the DNA molecules, causing the DNA to precipitate out of solution. Sodium ions (from the salt used in the extraction solution) also disrupt the DNA–water interactions, enhancing the precipitation of the DNA.

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Microsoft Word - 5 minute DNA Extraction.doc Author: Joshua Hall Created Date: 1/11/2012 8:36:55 PM ...

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DNA Extraction Lab: Strawberry. Background: The long, thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common, everyday materials. Strawberries are soft and easy to pulverize.

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In many DNA extraction protocols, the use of proteinase K is an important step because of its ability to digest harmful nucleases, but Even in the typical mouse-tail protocol, proteinase K is regularly used to inhibit harmful nucleases. And the addition of proteinase K occurs during the digestion step.

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Aug 09, 2010 · SYBR® Safe DNA gel stain (10,000X concentrate in DMSO). Use a narrow well comb. 1.2 Transfer 1µl of 50ng to 500ng of your genomic DNA sample(s) into clean labeled tube(s) and bring the total volume up to 4µl with 1X TE Buffer, pH 8.0. a. If the genomic DNA concentration is thought to be lower than 50ng/µl, then transfer 2-4µl of the sample(s).

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